Fig. 8.

Adoptively transferred NK cells can be detected in the liver, lungs, spleen and tumor of recipient mice. The presence of adoptively transferred NK cells in the spleens (gray bars), lungs (white bars) and livers (black bars) of mice receiving RKIK-4862 tumor and adoptively transferred, congenic CD45.1⁺ NK cells was investigated by flow cytometry. Adoptively transferred NK cells persist at detectable levels for at least 20 days post-transfer with respect to a the percentage of CD45.1⁺ cells in each organ and b the number of CD45.1⁺ circulating cells per milligram of organ. Data shown are average ± SEM from six mice from two independent experiments. c The presence of adoptively transferred NK cells in the tumor mass was investigated by flow cytometry to detect CD45.1⁺ NK cells following mechanical and enzymatic dissociation of the tumors from mice receiving RKIK-4862 tumor and adoptively transferred, congenic CD45.1⁺ NK cells (black bars) or RKIK-4862 alone (white bars) at day 17, post-transfer. Adoptively transferred NK cells could be detected in the tumor at this time point. p2 < 0.01 (NK cell-treated vs. non-treated groups). Data shown are average ± SEM from five mice per group