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. 1998 Nov;72(11):8921–8932. doi: 10.1128/jvi.72.11.8921-8932.1998

FIG. 10.

FIG. 10

PDGF β receptor tyrosine phosphorylation and complex formation in Ba/F3 cells. (Top) CHAPS extracts (900 μg of protein) from Ba/F3-derived cell lines expressing the murine PDGF β receptor either alone (−) or with the wild-type E5 protein (WT) or the indicated position 17 mutants were immunoprecipitated with anti-PDGF receptor antibody. Proteins were resolved by electrophoresis, transferred to membranes, and probed with antiphosphotyrosine antibody for detection of tyrosine-phosphorylated receptors. The figure is a composite of two independent immunoprecipitations, but the positive (WT) and negative (−) controls processed in parallel were included with both sets of immunoprecipitations. (Bottom) CHAPS extracts (1,500 μg of protein) from Ba/F3-derived cell lines were immunoprecipitated with anti-E5 antibodies. Precipitated proteins were resolved by electrophoresis and transferred, and membranes were probed with anti-PDGF receptor antibody for the detection of receptors associated with the E5 protein. Bands corresponding to the mature (m) and precursor (p) forms of the PDGF β receptor are indicated by arrows at the right, and the sizes of markers (in kilodaltons) are shown on the left.