Fig. 1.
Expression of myeloid/lymphoid markers CD1a, CD14, CD123, CCR7 and HLA-DR on CD83+ cells obtained from tumor-affected lung. Lung tissues were obtained from patients who underwent surgical resection of primary lung cancer. Tissue samples were minced and single-cell suspensions generated by digestion with collagenase type VIII (0.56 mg/ml; Sigma, St Louis, MO, USA) during 120 min incubation at 37°C. Cell suspensions (2 × 105 cells) were labeled with each of the various specific fluorescent antibodies (CD1a, CD14, CD83, CD123, CCR7 and HLA-DR) (Caltag Laboratories, Burlingame, California or BD Bioscience, Chicago, Illinois or R&D Systems, Minneapolis, Minnesota, USA), and analyzed in a FACSCalibur cytometer (Becton Dickinson, San Jose, California, USA) with the CellQuest software (Becton Dickinson, San Jose, California, USA)