Fig. 1.

The expression of transmembrane and secretory TNFα by transfected cells. a TNFα levels measured by ELISA from the 24 h cultured supernatants of 1×10⁶ cells stably transfected with various TNFα constructs. b Transmembrane TNFα on cell surface of transduced H22 tumor cells was analyzed by FACS. The cultured transfected H22 tumor cells were harvested and stained with anti-TNFα mAb and FITC-labeled goat anti-mouse IgG. At least 20,000 cells were counted. Data is representative of three independent experiments