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. 2006 Mar 23;55(12):1470–1479. doi: 10.1007/s00262-006-0150-x

Fig. 3.

Fig. 3

CD4+/CD8+ lymphocytes infiltration in TNFα and its mutants modified tumors. Indirect immunoenzymatic staining of frozen sections of tumors containing TNFα gene or its mutants transductants mixed with parental tumor cells at E/T of 5:1 was performed with specific primary antibodies (a goat anti-mouse CD4 or CD8 antibody) and the biotin-conjugated anti-goat IgG secondary antibody. Data are represented as mean ± SD. **P<0.01