Table 2.

Cytotoxicity of H22 cells transfected with TNF-α and its mutants in vitro^a

	H22/neo:H22	H22/TNFΔ1-12:H22	H22/TNF-IL2-SIG:H22	H22/wt-TNF:H22
E/T=5:1	2.0±1.22b	43.0±6.04	40.0±6.98	38.0±5.85
E/T=1:1	1.8±1.64	17.0±4.52	22.0±5.06	15.0±6.56
E/T=1:5	1.6±0.97	8.2±4.38	9.0±4.78	6.0±3.02
1:5/5:1c		18.6%	22.5%	15.79%

^aTNFα and its mutants transduced and non-transduced H22 tumor cells were mixed at different E/T ratios as shown in the table. The mixed cells (4×10⁵/100 μl/well) were seeded in 96-well microtiter plates and incubated for 48 h. Viability of cells was measured by staining with MTT. Data are represented as mean ± SD

^bThe numbers in columns are cytotoxicity rates (%)

^cCytotoxicity of tranductant at E/T of 1:5 is the percentage of its cytotoxicity at E/T of 5:1