Table 3.

The inhibitory rate of tumor growth by TNFα or its mutants in vivo^a

	H22/neo:H22	H22/TNFΔ1-12:H22	H22/TNF-IL-2SIG:H22	H22/wt-TNF:H22
E/T=1:0	1.5±0.40b	83.0±8.04	82.0±7.96	73.0±6.62
E/T=5:1	2.0±1.00	68.0±5.72	72.0±5.89	57.0±5.12
E/T=1:1	1.8±0.67	54.0±3.87	71.0±6.05	54.0±3.76
E/T=1:5	1.6±1.23	14.0±4.28	48.0±4.07	35.0±5.06
1:5/5:1c		20.58%	66.67%	61.4%

^aTNF and its mutants transduced and non-transduced H22 tumor cells were mixed at different E/T ratios as shown in the table. 2.5×10⁵ mixed tumor cells were injected s.c. to syngeneic BALB/C mice. Tumor size was measured every 3 days. The inhibitory rate of tumor growth was calculated with the following formula: (1-mean tumor size on 21 days of TNF transductants/ mean tumor size on 21 days of non-transfected control) ×100%. Data are represented as mean ± SD

^bThe numbers in columns are inhibitory rates (%) of tumor growth

^cThe inhibitory rate of tumor growth by TNFα or its mutants at E/T 1:5 is the percentage of their action at E/T 5:1