Table 1.
Characteristics of the different available closed systems for monocyte enrichment and subsequent DC generation
| Adherence | Positive selection | Negative selection | Elutriation | |
|---|---|---|---|---|
| Cost | Low | High | High | Low |
| Monocyte puritya | ±60% | 91–99% | 8–75% | 55–90% |
| Main monocyte contaminantsb | B, NK cells | / |
NK cells Granulocytes |
Granulocytes (especially neutrophils) |
| Monocyte recovery | ND | 27–100% | 43–97% | 53.3–88.2% |
| Closed culture recipient |
Cell FactoriesTM Gas-permeable bags |
Gas-permeable bags | Gas-permeable bags | Gas-permeable bags |
| Monocyte activation | Possible | Possible | No | No |
| DC purityc | 34–98% | 59.5–98% | 31–86% | 62–98% |
| DC yield (PBMC) | 2.7–20% | 1–2% | 4.8–13% | 5–12% |
| DC yield (monocytes) | 12–68% | 4–41% | 16–95.1% | 20–100% |
| References | [17, 57, 72, 108, 135, 167, 172, 183, 189] | [10, 52, 57, 66, 108] | [57, 108, 135, 172, 197] |
[2, 18, 66, 149, 167, 178, 197] Dr. H. Vrielink (personal communication) |
a Monocyte purity was assessed by flow cytometry using the monocytic marker CD14
b Contaminants of monocyte preparations were assessed by flow cytometry using lineage-specific antibodies
c DC purity was evaluated by FSC/SSC characteristics or by CD83 positivity