Fig. 3.

Induction of Top2α-specific immune responses by Top2αCRNA/DC vaccination. a IFN-γ-secreting T cells specific for Top2α in splenocytes from mice vaccinated with Top2αCRNA/DC. Splenocytes stimulated with Top2αCRNA/DC and MC-38TL/DC as Top2α-expressing cells. IFN-γ-secreting T cells were measured by ELISPOT assay. b IFN-γ-secreting T cells specific for Top2α in CD4⁺ or CD8⁺ T cells sorted from splenocytes of mice vaccinated with Top2αCRNA/DC. CD4⁺ and CD8⁺ T cells were stimulated by Top2αCRNA/DC. Results are given as the mean number of IFN-γ spots per 5 × 10⁴ cells from individually tested mice ±SD. The statistical significance was evaluated using a t test. *P < 0.05 (DC vs. Top2αCRNA/DC). The data are representative of three independent experiments