Table 1.

PCR probes and primers (fluorophore)

RNA target	Probe/primer	Sequence
28 S	Probe	5′-(HEX)-AGGACCGCTACGGACCTCCACCA-(TAMRA)-3′
	F	5′-GGCGAAGCCAGAGGAAACT-3′
	R	5′-GACGACCGATTTGCACGTC-3′
IL-2	Probe	5′-(FAM)-ACTGAGACCCAGGAGTGCACCCAGC-(TAMRA)-3′
	F	5′-TTGGAAAATATCAAGAACAAGATTCATC-3′
	R	5′-TCCCAGGTAACACTGCAGAGTTT-3′
IL-4	Probe	5′-(FAM)-AGCAGCACCTCCCTCAAGGCACC-(TAMRA)-3′
	F	5′-AACATGCGTCAGCTCCTGAAT-3′
	R	5′-TCTGCTAGGAACTTCTCCATTGAA-3′
IL-6	Probe	5′-(FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-(TAMRA)-3′
	F	5′-GCTCGCCGGCTTCGA-3′
	R	5′-GGTAGGTCTGAAAGGCGAACAG-3′
IL-8	Probe	5′-(FAM)-TCTTTACCAGCGTCCTACCTTGCGACA-(TAMRA)-3′
	F	5′-GCCCTCCTCCTGGTTTCAG-3′
	R	5′-TGGCACCGCAGCTCATT-3′
IL-10	Probe	5′-(FAM)-CGACGATGCGGCGCTGTCA-(TAMRA)-3′
	F	5′-CATGCTGCTGGGCCTGAA-3′
	R	5′-CGTCTCCTTGATCTGCTTGATG-3′
IL-12β	Probe	5′-(FAM)-CTGAAAAGCTATAAAGAGCCAAGCAAGACGTTCT-(TAMRA)-3′
	F	5′-TGGGCAAATGATACGGTCAA-3′
	R	5′-CAGAGTAGTTCTTTGCCTCACATTTT-3′
IL-13a	Probe	5′-(FAM)-CTGCCACAGTGCTGGACAACATGACCG-(TAMRA)-3′
	F	5′-CAAGGATCGGAAGCTGTCAGAG-3′
	R	5′-GGCGGGCAGTTCGTCATG-3′
IL-18	Probe	5′-(FAM)-CCGCGCCTTCAGCAGGGATG-(TAMRA)-3′
	F	5′-AGGTGAAATCTGGCAGTGGAAT-3′
	R	5′-ACCTGGACGCTGAATGCAA-3′
CTLA-4a	Probe	5′-(FAM)-TTGTCTTCTCTGAATCGCTTTGCCCACG-(TAMRA)-3′
	F	5′-CAGCATCATCATCTCAGCCATTG-3′
	R	5′-GCATTTTCACATAGACCCCAGTAG-3′
GPR83a	Probe	5′-(FAM)-TCCGCCACCAGCCTGTTCATCGTCA-(TAMRA)-3′
	F	5′-CGTCATCATCAAGAGCAAACGC-3′
	R	5′-ACAAAACGAGCCAGTGTAAAAGG-3′
IFN-γ	Probe	5′-(FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA)-3′
	F	5′- GTGAAGAAGGTGAAAGATATCATGGA-3′
	R	5′-GCTTTGCGCTGGATTCTCA-3′
SMAD7a	Probe	5′-(FAM)-TCCCAGTAAGCCACCACGCACCAGT-(TAMRA)-3′
	F	5′-GCTCTCAGATTCTCAAGTTATTCAGG-3′
	R	5′-CCGACCCACACGCATCTTC-3′
TGFβ	Probe	5′-(FAM)-ACCCAAAGGTTATATGGCCAACTTCTGCAT-(TAMRA)-3′
	F	5′-AGGATCTGCAGTGGAAGTGGAT-3′
	R	5′-CCCCGGGTTGTGTGTTGGT-3′

F forward, R reverse

^aDesigned during this work