Fig. 1.

a Direct antigen binding to ganglioside GM2 of anti-GM2 and anti-GM2-T15 detected by ELISA assay. GM2 ganglioside was coated onto the ELISA plate. An anti-HER2 antibody (Herceptin) was applied as non-specific control. Three independent experiments have been performed to determine the means and standard deviations. b GM2 ganglioside antigen was coated onto plates, antibody was added and serially diluted. An avidin-HRP conjugate was used to rule out Fc effects. Double asterisk, significant difference compared with anti-GM2 group, p < 0.01