Fig. 4.

CCL2 induces the migration of CD4⁺CD25⁺FoxP3⁺ Tregs. CD4⁺ T cells were isolated from PBMCs of healthy donors (n = 3). Cells that migrated toward the chemokines in the supernatant (sup) were stained with fluorescent-conjugated antibodies to CD4, CD25, FoxP3. The percentage increase in numbers of Tregs over that of the control group (CD4⁺ T cells assayed with medium alone) was analyzed by flow cytometry. Bars standard error