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Confirmation of mass spectrometry results. Extracts of WM793 (lane 1) and Ma-Mel-27 (lane 2) cellular proteins were precipitated using VVL-agarose, separated by SDS-PAGE and blotted on PVDF membrane. In parallel, protein standards were resolved (lane 3). Immunodetection of nucleolin was performed using anti-human nucleolin mAb, clone 3G4B2
