Table 1.
ProPred-1 prediction of MHC class-I binding sites in nucleolin
| First position of binder | Sequence | ALLELE: HLA | Cell line |
|---|---|---|---|
| 168 | EIEPAAMKA | A1 | KNUD |
| 617 | NSEEDAKAA | ||
| 481 | SGESKTLVL | ||
| 95 | KTVTPAKAV | A2 | Ma-Mel-12 |
| 522 | KGYAFIEFA | Ma-Mel-04 | |
| 300 | TEPTTAFNL | ||
| 223 | VVPVKAKNV | ||
| 223 | VVPVKAKNV | A*0201 | WM115 |
| 63 | VVVSPTKKV | ||
| 522 | KGYAFIEFA | ||
| 95 | KTVTPAKAV | ||
| 493 | SYSATEETL | A24 | Ma-Mel-27 |
| 314 | NFNKSAPEL | Ma-Mel-8b | |
| 355 | SAEDLEKAL | ||
| 109 | KGATPGKAL | ||
| 391 | ARTLLAKNL | B14 | Ma-Mel-8b |
| 318 | SAPELKTGI | B*3501 | Ma-Mel-8b |
| 170 | EPAAMKAAA | Ma-Mel-12 | |
| 109 | KGATPGKAL | ||
| 75 | TPAKKAAVT | ||
| 632 | GEIDGNKVT | B*4403 | WM115 |
| 281 | KEMAKQKAA | KNUD | |
| 300 | TEPTTAFNL | ||
| 169 | IEPAAMKAA | ||
| 281 | KEMAKQKAA | B61 | Ma-Mel-27 |
| 297 | VEGTEPTTA | ||
| 169 | IEPAAMKAA | ||
| 632 | GEIDGNKVT | ||
| 407 | ELKEVFEDA | B62 | Ma-Mel-27 |
| 109 | KGATPGKAL | B7 | Ma-Mel-04 |
| Ma-Mel-12 | |||
| 216 | KGKKAAKVV | B*0702 | Ma-Mel-04 |
| 281 | KEMAKQKAA | Ma-Mel-12 | |
| 122 | GKKGAAIPA | ||
| 95 | KTVTPAKAV | ||
| 357 | EDLEKALEL | Cw*0301 | Ma-Mel-12 |
| 351 | VDFESAEDL | ||
| 493 | SYSATEETL | Cw*0401 | Ma-Mel-12 |
| 314 | NFNKSAPEL | ||
| 479 | TWSGESKTL | ||
| 391 | ARTLLAKNL | Cw*0602 | WM115 |
| 355 | SAEDLEKAL | Ma-Mel-12 |
N potential N-glycosylation sites, T potential O-glycosylation sites
The alleles of MHC class-I present on analysed cell lines: KNUD, Ma-Mel-04, Ma-Mel-8b, Ma-Mel-12, Ma-Mel-16, Ma-Mel-27 and WM115 were taken into consideration. All peptides having score greater than threshold score at 4% were considered as predicted binders for selected MHC alleles