Fig. 5.
Preventive and therapeutic effect of MZF in a subcutaneous colon-26 tumor model. a, b Preventive effect of MZF. BALB/c mice were treated with three s.c. injections (a week apart) of 2 × 105 DCs prepared as follows: DC, Lysate-DC, LPS + Lysate-DC, or MZF + Lysate-DC. A week after the final DC injection, colon-26 cells (1 × 105 cells/mouse) were s.c. inoculated. The tumor growth (a) and survival rates (b) were monitored (control, n = 14; other groups, n = 7). c, d Therapeutic effect of MZF. Colon-26 cells (1 × 105 cells/mouse) were s.c. inoculated into BALB/c mice on day 0. On days 3, 10, and 17, mice were treated by s.c. injections of 2 × 105 DCs. The tumor growth (c) and survival rates (d) were monitored (control, n = 14; other groups, n = 7). a, c The Mann–Whitney U test was used to analyze the statistical significance of tumor growth on day 21. *p < 0.05 versus all other groups; # p < 0.05 versus control. b, d Survival rates are represented using Kaplan–Meier curves