Table 1.
Maturation marker | MZF concentration (μg/ml) | LPS | |||
---|---|---|---|---|---|
0 | 100 | 200 | 400 | ||
CD80 | |||||
Percentage of positive cells | 31.96 ± 0.58 | 34.91 ± 0.62* | 36.34 ± 0.36* | 44.35 ± 0.38* | 66.33 ± 0.39* |
MFI | 74.68 ± 2.08 | 70.28 ± 2.20 | 75.04 ± 1.91 | 102.88 ± 2.97* | 165.98 ± 4.43* |
CD86 | |||||
Percentage of positive cells | 35.20 ± 2.94 | 42.49 ± 0.35 | 47.16 ± 1.14* | 61.29 ± 8.47* | 72.97 ± 0.78* |
MFI | 55.56 ± 6.81 | 88.70 ± 4.04* | 92.01 ± 2.03* | 109.76 ± 2.26* | 186.50 ± 2.25* |
CD83 | |||||
Percentage of positive cells | 10.13 ± 0.38 | 13.06 ± 1.13 | 17.94 ± 0.43* | 24.69 ± 0.59* | 24.83 ± 0.49* |
MFI | 4.98 ± 0.08 | 5.99 ± 0.25* | 6.43 ± 0.20* | 8.72 ± 0.54* | 9.50 ± 0.59* |
MHC class II | |||||
Percentage of positive cells | 28.25 ± 0.43 | 29.06 ± 0.71 | 32.69 ± 0.69* | 43.95 ± 0.24* | 40.17 ± 0.22* |
MFI | 131.59 ± 2.85 | 143.05 ± 2.90* | 144.75 ± 3.17* | 179.11 ± 1.97* | 182.85 ± 3.97* |
DCs (1 × 106 cells/ml) were stimulated with MZF (0–400 μg/ml) or LPS (100 ng/ml) for 24 h. The values are percentages of DCs (CD11c+ cells) expressing indicated markers and the mean fluorescence intensity (MFI). Values are expressed as mean ± SE (n = 3)
* p < 0.05 versus control