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. 1998 Nov;72(11):8933–8942. doi: 10.1128/jvi.72.11.8933-8942.1998

FIG. 3.

FIG. 3

gE requires gI to reach the surface of epithelial cells. HEC-1A cells were infected with either Ad(E1−)gE (A and D), Ad(E1−)gE plus Ad(E1−)gI (B and E), or Ad(E1−)gD (C and F) for 48 h. The cells were fixed with paraformaldehyde, either permeabilized with 0.2% TX100 (A to C) or left unpermeabilized (D to F), and then incubated with MAb 3114, specific for gE (A, B, D, and E), or MAb DL-6, specific for gD (C and F). The cells were washed, incubated with FITC-coupled goat anti-mouse antibodies, washed again, mounted on coverslips, and viewed under a Nikon epifluorescence microscope.