Fig. 4.

Chemotaxis of HL TIL to purified chemokine. Isolated TIL from HL biopsies were tested for chemotaxis to recombinant human CXCL12 (200 ng/ml), CCL21 (500 ng/ml) and CXCL9 (500 ng/ml). All HL cases were NS subtype except HL3 and HL9 which were MC. Cryopreserved PBMCs derived from a healthy donor were thawed and used as positive controls. Results are shown as chemotactic index values calculated by dividing the number of cells migrating in response to specific chemokine by the number of cells migrating in medium alone (spontaneous migration). The mean value for spontaneous migration was 384 cells. Data represent the mean of triplicate tests ± SD