Fig. 3.
Effect of αCD40 on B16-hsp72.1 tumor growth in vaccinated and non-vaccinated mice: role of T and NK cells. C57BL/6 mice were injected s.c. with 106 γ-irradiated B16-hsp72.1 cells on days −42 and −21. On day 0, these vaccinated mice (a) and naïve mice (b) were injected s.c. with 7.5 × 105 B16-hsp72.1 tumor cells. αCD40 (0.5 mg) or rat IgG were injected i.p. on days 5, 12 and 19. αCD40-treated mice were depleted of T cells or NK cells as described in “Materials and methods”. Control mice received rat IgG instead of anti-CD4/CD8 mAbs. Tumor volumes are presented as Mean ± SEM. *P < 0.05