Fig. 2.

Treatment of mice with established s.c. B16-F10 melanoma tumors. a Mice were treated peritumorally with tumor specific peptide phage (WDC-2), non-tumor specific phage (Fab) (1 × 10¹¹ pfu/cfu in 100 μl PBS), or PBS alone (100 μl) for up to 2 weeks. WDC-2 phage (filled circle) was able to control tumor growth for 2 weeks compared to the Fab-phage (filled diamond) and PBS (filled triangle) treatment. The difference in the mean tumor volume on day 13, comparing WDC-2 treated to Fab-phage and PBS treated mice was significantly different as determined by Student’s t-test (star indicates P < 0.01). The graph shows tumor development from treatment start (arrow). Horizontal bars represent mean values. A minor, but not significant (P = 0.057) increase of survival in mice treated with tumor specific phage was also observed (b), as determined by log-rank analysis. The data is representative of two experiments