Fig. 3.

Treatment of mice with established s.c. B16/A2K^b melanoma tumors. a Mice were treated with tumor specific phage (Fab and WDC-2), wild-type M13 (1 × 10¹¹ pfu/cfu in 100 μl PBS) or PBS alone (100 μl) for 2 weeks. The numbers of long-term surviving mice with complete tumor regression are indicated in parenthesis. The difference between tumor free mice, comparing those who received tumor specific phage versus wild-type phage, was statistically significant (star indicates P < 0.01), as determined by Chi-square testing. b Tumor specific phage treatment significantly increases survival compared to control phage treatment. Survival was monitored 2- times per week, and plotted as a Kaplan–Meier survival curve based on the experiment from (a). Mice were sacrificed when the tumors reached a volume of 250 mm³. WDC-2 and HLA-A2 specific phage treatment significantly increased survival of mice with established tumors (two stars indicate P < 0.0001) compared to wild-type phage treatment, as determined by log-rank analysis