Fig. 6.

Activated R-Ras increased migration of cervical epithelial cells via mTOR. Monolayers of C33A cells transfected with activated R-Ras (R-Ras87L), or activated PI 3-K (PI 3-K p110), were grown on collagen I-covered six-well plates, and scratched (wounded) with a pipette tip (0 h). Cells were then covered with fresh medium and monolayers were allowed to recover. Wound area was photographed 24 and 48 h later. Some cells were cultured in the presence of 5 nM Rapamycin. Data are from one of three different experiments that yielded similar results