Fig. 9.

Assessing the roles of VEGF and the Tie-2 pathway on tumor skewing of CD34⁺ cells into CD31⁺ endothelial cells. CD34⁺ cells were seeded under conditions to support myeloid cell growth in the presence or absence of 40% tumor-conditioned medium which had been admixed with neutralizing VEGF antibody or sTie-2-Fc. After 7 days, cells were detached and seeded onto Matrigel-coated wells. After 24 h, CD31⁺ cells were detected by immunocytochemical staining