Fig. 1.

Flow cytometry analysis of MISIIR ECD expressing HEK:MISIIR“+”. Following the stable transfection of HEK cells with the pDisplay vector encoding the MISIIR ECD gene and a N-terminal HA tag, specific expression of the HA tag and the MISIIR ECD on the cell membrane was determined by flow cytometry. a Expression of HA-tagged MISIIR ECD on the surface of HEK::MISIIR“+” cell line (unfilled line) as compared to parental HEK293 cells (filled peak). Expression was detected with the FITC conjugated 3F10 anti-HA tag MAb. b Specific expression of MISIIR ECD on HEK::MISIIR“+” (unfilled line) vs. parental HEK293 cells (filled peak) as detected by an anti-MISIIR ECD monoclonal antibody [44] and a secondary FITC conjugated anti-mouse IgG. The secondary antibody failed to bind to either cell line alone (data not shown)