Fig. 2.

SCLC cells are deficient for Tapasin in addition to TAP. Protein extracts from SCLC cell lines, before and after treatment with IFNγ, were analysed by western blotting with rabbit anti-sera specific for a TAP1 and TAP2, and b Tapasin. Control cell lines included for the TAP blot, a standard TAP-positive lymphoblastoid cell line (B-LCL), and TAP-negative T2 cells, and for the Tapasin blot the Tapasin negative .220 cell line and its tapasin-transfected counterpart (.220:Tpn). Replicate blots were probed for actin as a loading control. c Flow cytometric analysis of expression of HLA-A2 on SCLC cell lines NCI-H69 and NCI-H345 before and after treatment with IFNγ, as determined by staining cells with monoclonal antibody BB7.2