Fig. 1.

CD14 and CD1a expressions by monocytes in the presence or absence of K562 supernatants. Monocytes isolated from healthy volunteers were incubated with IL-4 and GM-CSF, in the presence or absence of 5, 10 and 20% final volume of K562 supernatant (SN K562) for 5 days. After this period, CD14 and CD1a expressions were analyzed by flow cytometry. Representative histogram (a) shows CD14 and CD1a expressions in a control differentiation. Data in bars (b) show CD14 and CD1a expressions of monocytes cultured in medium (white bar), with IL-4 and GM-CSF (black bar) and in the presence of SN K562 (gray bars). The result shows the mean ± SEM of at least three independent experiments. a Compared with monocytes without IL-4 and GM-CSF, b compared with monocytes cultured with IL-4 and GM-CSF. *Significantly different (P < 0.05), **Significantly different (P < 0.01)