Fig. 3.

Recognition of cells transfected with MAGE-4.COS-7 cells were transiently transfected with a MAGE-4a or a MAGE-1 coding sequence inserted in expression vector pcDNAI/Amp and an HLA–A24 coding sequence inserted in expression vector pcDNA3. Transfections were performed with 15,000 COS-7 cells, 50 ng of each cDNA and 1 μl of Lipofectamine. One day after transfection, 3,000 CTL 13 were added to the transfected cells. IFN-γ production was measured by ELISA after overnight co-culture