Fig. 4.

Anti-TGF-α-specific T cell lines are MHC class II restricted. The specificity of T lymphocytes expanded from PBMC with TGF-α peptide 121–140 (a), 71–90 (b) and 131–150 (c) was evaluated as described in Fig. 3. Data for IFN-γ ELISPOT and GM-CSF secretion assays are presented. T cell line specificity was based on criteria described in Fig. 3 legend. D. The T cell line specific to TGF-α peptide 121–140 was co-cultured with allogeneic or autologous APC pulsed with the relevant peptide with or without antibodies blocking antigenic presentation mediated by MHC class I or class II molecules. TNTC to numerous to count, auto autologous, allo allogenic