Fig. 2.
Competition experiments. a, b Competition ELISAs with immobilized asialoglycophorin (aGP). a Binding of mouse anti-TF antibody A78-G/A7 was inhibited by different amounts of purified scFv(1aa). Bound A78-G/A7 was detected with POD-labeled anti-mouse Ig antiserum. b Binding of scFv(1aa) was inhibited by dilutions of supernatants from two mouse anti-TF antibodies, A78-G/A7 (grey bars) or NEMOD-TF2 (black bars). Binding of scFv(1aa) was detected with rabbit anti-myc antiserum followed by POD-labeled anti-rabbit Ig antiserum. c Analysis of binding kinetics by surface plasmon resonance on a BIAcore biosensor instrument under competition conditions. Overlay plot of sensorgrams of the dissociation of scFv(1aa) from aGP coupled to a dextran chip as influenced by different TF-carrying molecules: a control (PBS); b monomeric TFα (200 mM); c aGP (50 μM); d TFα coupled to PAA (multimeric, 50 μM)