Table 2.

Scatchard analysis of cell binding assays

	N a	Cell line	Binding sitesb	% Bindingc	K d (M)
ScFv(0aa)	4	NM-D4	4.8 × 106 ± 3.3 × 106	37.8 ± 6.2	1.0 × 10−7 ± 4.6 × 10−8
	3	ZR-75−1	2.5 × 106 ± 2.0 × 105	10.3 ± 2.9	4.2 × 10−7 ± 1.6 × 10−7
ScFv(1aa)	9	NM-D4	8.1 × 106 ± 5.5 × 106	17.2 ± 7.5	5.3 × 10−7 ± 1.8 × 10−7
	3	ZR-75-1	2.2 × 106 ± 1.2 × 106	2.3 ± 0.6	1.5 × 10−6 ± 7.4 × 10−7
	2	K-562	–	0.8	–

^aNumber of individual experiments

^bNumber of binding sites per cell. Determined by extending the trend line of the Scatchard plot to the abscissa

^cPercent of the antibodies bound to the cell pellet