Fig. 7.

Cytotoxicity assays. a NK cytotoxicity assays. NK cells that were obtained from splenocytes of mice 7 days after the last immunization with pIL-18 and pAPOPTIN plasmids were used as effector cells in an LDH-release assay. In this assay, YAC-1 cells were used as target cells. b CTL cytotoxicity assays. T cells that were derived from splenocytes of mice immunized with pIL-18 and pAPOPTIN plasmids were used as effector cells in an LDH-release assay, in which LLC tumor cells were used as target cells. c To confirm that T cell cytotoxicity was LLC tumor-specific, we also included another tumor cell line of C57BL/6 origin, B16, as a target control in this experiment. B16-specific CD8⁺ T cells were used as positive controls. Values represent the mean of triplicate from two experiments. Standard deviation (SD) of each point is less than 5% of the mean value