Fig. 3.

Cox-2 independent ROS induction in Ramos B lymphoma cells. DCF fluorescence intensity was analyzed by imaging flow cytometry in Ramos B lymphoma cells either a untreated or treated with b SC-58125 (20 μM) or c OSU03012 (5 μM). 7-AAD (cell impermeable) and Draq5 (cell permeable) dyes were used to analyze for cell viability. SC-58125 and OSU03012 showed an increased percentage of ROS positive cells compared to untreated. SC-58125 induced ROS in a larger proportion of live cells compared to OSU03012. A subset of early apoptotic OSU03012 treated cells (R2 gate-Draq5 and 7-AAD double positive) showed the largest increase in DCF fluorescence