Fig. 1.

Pharmacokinetic profile, body temperature and T cell activation markers after single intravenous injection of muS110. Female BALB/c mice (n = 20) received injection of 5, 15 or 50 μg/kg muS110 or 500 μg/kg control BiTE antibody. a Serum concentration of muS110 (n = 4); b body temperature (n = 20) as determined before and 0.5, 1, 2, 4, 6, 8, 10, 24 h after BiTE antibody injection. c Activation of T cells isolated from mesenteric lymph nodes before and 1, 2, 4, 10, 24 h after muS110 administration was determined by analysis of c CD69, or d CD25 expression using flow cytometry (n = 4). Error bars indicate standard error of the mean. The dashed lines are results with a control BiTE antibody solely sharing the CD3-binding moiety with muS110