Table 2.

Histopathological examination of EpCAM-positive tissues from mice treated with muS110

EpCAM positive tissue	Score of EpCAM expression	Number of analyzed animals vehicle	Number of analyzed animals MuS110	MuS110 specific histopathological findings
Caecum	2	32	30	None
Colon	4	32	30	None
Eyes	2	32	30	None
GI tract other	2–4	32	30	None
Kidneys	1	32	30	None
Liver	1	32	30	None
Lung and bronchi	1	32	30	None
Lymph nodes mandibular	1	32	30	None
Lymph nodes mesenteric	1	32	30	None
Oesophagus	2–4	32	30	None
Pancreas	3	32	30	None
Pituitary	2–3	32	30	None
Salivary glands	2–4	32	30	None
Thymus	1	32	30	None
Parathyroids	3–4	23	22	None
Ureters	2	32	30	None
Urinary bladder	2	32	30	None
Male reproductive system
Prostata	3	16	16	None
Testes	1	16	16	None
Female reproductive system
Mammary area (caudal)	2	16	14	None
Uterus	2–4	16	14	None
Uterine cervix	2	16	14	None
Vagina	2	16	14	None

Sixteen male and female BALB/c mice per group received daily intravenous injections of vehicle for 35 days or of 10 μg/kg/day muS110 for 7 days followed by 100 μg/kg/day muS110 for 28 days. Samples of tissues were fixed, dehydrated, embedded in paraffin wax, sectioned at approximately 4–5 µm thickness, and stained with H&E. All slides were inspected and evaluated by a certified pathologist. MuS110 specific findings for EpCAM positive tissues (score of stain ≥ 1) are shown. The scoring of tissues for relative intensity and frequency by immunohistochemical analysis for EpCAM expression is described in Amann et al. [4]. The EpCAM expression score from 1–4 is as follows: 1 light staining and/or low percentage of cells stained; 2 moderate staining and/or moderate percentage of cells stained; 3 medium-strong staining and/or or medium percentage of cells stained; 4 intense staining and/or or high percentage of cells stained

GI tract other includes duodenum, ileum and jejunum