Fig. 1.

Immunodominant CD8⁺ T cell response in naturally occurring NY-ESO-1-seropositive EOC patient. CD8⁺ cells were co-cultured with Ade-ESO infected APC for 14 days. a IFN-γ production in HLA-A2/ESO_157–165 multimer positive or negative CD8⁺ cells was analyzed against autologous T-APC pulsed with pooled peptides, ESO_157–165 or Flu-MA_58–66 by intracellular staining. b The number of IFN-γ producing CD8⁺ cells was tested by ELISPOT assay against autologous T-APC pulsed with 17 overlapping NY-ESO-1 20–25-mer peptides, ESO_157–165 or Flu-MA_58–66 peptides. c CD107 and IFN-γ expression of CD8⁺ cells were examined by intracellular staining upon stimulation with ESO_119–143 or Flu-MA_58–66 peptides