Fig. 3.

Identification of the optimal epitope within ESO_119–143 recognized by HLA-A68-restricted B01 clone. a B01 clone was stimulated with B05-EBV-B cells pulsed with overlapping 9- or 10-mer peptides between ESO_119–143, and cytoplasmic IFN-γ productions were determined by intracellular staining. b B01 clone was cocultured with autologous T-APC pulsed with the indicated peptides, and CD107 expression and IFN-γ and TNF-α productions were examined by intracellular staining. c The affinity to peptides were analyzed by intracellular staining against autologous T-APC pulsed with the different concentration of ESO_119–143, ESO_128–136 and ESO_127–136 peptides. d CD8⁺ cells from HLA-A68^+ve patients who was NY-ESO-1 seropositive (patient B03), and was seronegative but vaccinated with recombinant fowlpox-NY-ESO-1 and recombinant vaccinia-NY-ESO-1 (patient B04) were presensitized with ESO_119–143 peptide for 14 days, and IFN-γ secretion were determined by ELISPOT assay against the indicated peptide pulsed autologous T-APC