Fig. 4.

Detection of immunodominant CD8⁺ T cell response in HLA-A2^+ve EOC patient with spontaneous antibody response to NY-ESO-1. CD8⁺ cells were presensitized with pooled NY-ESO-1 overlapping peptides for 14 days. a CD8⁺ cells were analyzed for reactivity against autologous T-APC pulsed with pooled peptides, ESO_157–165 or Flu-MA_58–66 peptide by intracellular staining. b The responses of CD8⁺ cells were tested by IFN-γ ELISPOT assay against autologous T-APC pulsed with 17 overlapping NY-ESO-1 20–25-mer peptides. c CD107 and IFN-γ expression of CD8⁺ cells was examined by intracellular staining upon stimulation with autologous T-APC pulsed with or without ESO_119–143