Fig. 6.

Identification of the minimal epitope of ESO_119–143 recognized by HLA-Cw15-restricted B02 clone. a B02 clone were stimulated with autologous T-APC pulsed with overlapping 9- or 10-mer peptides in ESO_119–143, and cytoplasmic IFN-γ productions were determined by intracellular staining. b B02 clone was cocultured with autologous T-APC pulsed with the indicated peptides, and CD107 expression and IFN-γ and TNF-α productions were examined by intracellular staining. c The affinity to peptides were analyzed in ELISPOT assay against autologous T-APC pulsed with the different concentration of ESO_119–143, ESO_127–135, ESO_128–136 and ESO_127–136 peptides. d CD8⁺ cells in NY-ESO-1 seropositive and HLA-Cw15^+ve EOC patients were presensitized with ESO_127–135 peptide for 20 days, and IFN-γ and CD107 expression was examined by intracellular staining against the indicated peptide-pulsed autologous T-APC