Extended Data Fig. 8. Bulk RNA-seq and cytokine production analysis of WT or Trem2−/− BV2 cells.
a) Heat map of nonfoamy macrophages comparing top enriched WT and Trem2-/- genes. b) Heat map of foamy macrophages comparing top enriched WT and Trem2-/- genes. c) Normalized enrichment scores for top pathways associated with WT BV2 compared to Trem2-/- BV2 in media alone (nonfoamy) or following foamy differentiation. Significant pathways were determined using Weighted-Kolmogorov-Smirnov (WKS) test. d) Heat map of nonfoamy and foamy macrophages comparing matrix metalloprotease genes from WT and Trem2-/- BV2. e) Cytokine supernatant analysis from cultured WT or Trem2-/- cells cultured with either media alone or media with 20 mg/ml soluble cholesterol overnight (n = 3 biological replicates/group). Data are mean ±S.E.M.