Extended Data Fig. 9. Trem2-/- peritoneal macrophages recapitulate BV2 phenotypes.
a) WT or Trem2-/- peritoneal macrophages were differentiated in media control, media with 20 µg/mL or 80 µg/mL soluble cholesterol to induce foamy macrophage formation. Cell supernatant was assessed for cytotoxicity by LDH assay after 16 hours (n = 5 biological replicates/group). Data are mean ± S.E.M. Two-tailed ANOVA, P = * < 0.05. b) WT or Trem2-/- peritoneal macrophages were differentiated in media control or media with 20 µg/mL soluble cholesterol, then cultured with irradiated, cell trace violet (CTV) labeled splenocytes for 2 hours. Percentage of efferocytotic cells were determined by the % of peritoneal macrophages that were positive for CTV labeled splenocytes (n = 5 biological replicates/group). Data are mean ± S.E.M. Two-tailed ANOVA, P = *** < 0.001, ****<0.0001. c) WT or Trem2-/- peritoneal macrophages were differentiated in media control or media with 20 µg/mL soluble cholesterol, then assessed for activation of ER stress response by sXBP1 levels by flow cytometry. Tunicamycin was used as a positive control (n = 5 biological replicates/group). Data are mean ± S.E.M. Two-tailed ANOVA, P = *** < 0.001.