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. 2023 Oct 30;2(11):1015–1031. doi: 10.1038/s44161-023-00354-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, Confocal micrograph showing CD68 staining (green) and DAPI (blue) for macrophage area in Cntl or Trem2-deficent mice after 16-week TAM-HFD feeding. Representative image from two independent experiments. b, Quantification of CD68⁺ macrophage area per section in 8-week or 16-week TAM-HFD samples (n = 5 mice per group). Data are mean ± s.e.m. Student’s t-test, *P < 0.05 and **P < 0.01. c, Quantification of the percentage of plaque that is macrophages (CD68⁺) in 8-week or 16-week TAM-HFD samples (n = 5 mice per group). Data are mean ± s.e.m. Student’s t-test. d, Confocal micrograph showing Ki67 staining (magenta) and CD68 staining (green) for proliferation in Cntl or Trem2-deficient mice after 16-week TAM-HFD feeding. Representative image from two independent experiments. e, Quantification of Ki67⁺ macrophages (CD68⁺) per section in 8-week or 16-week TAM-HFD samples (n = 5 mice per group for 8-week TAM-HFD, n = 7 mice per group for Cntl 16-week TAM-HFD and n = 6 mice per group for 16-week TAM-HFD Trem2^ΔMФ). Data are mean ± s.e.m. Student’s t-test, *P < 0.05 and **P < 0.01. f, Confocal micrograph of TUNEL staining (magenta) and CD68 staining (green) for detection of dying cells within atherosclerotic lesions after 16-week TAM-HFD feeding. Representative image from two independent experiments. g, Quantification of TUNEL⁺ macrophages (CD68⁺) per section in 8-week or 16-week TAM-HFD samples (n = 6 mice per group for Cntl 8-week TAM-HFD, n = 7 mice per group for Trem2^ΔMФ 8-week TAM-HFD, n = 7 mice per group for Cntl 16-week TAM-HFD and n = 7 mice per group for Trem2^ΔMФ 16-week TAM-HFD). Data are mean ± s.e.m. Student’s t-test, *P < 0.05 and **P < 0.01.

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