FIG. 1.

Nuclear factor binding sites in the M-MuLV enhancers. A schematic representation of the M-MuLV LTR with the nuclear protein binding sites (bracketed sequences) and sequence-specific DNA proteins known to bind to them (in boxes) in one copy of the 75-bp direct repeats is shown. (These data are taken from Manley et al. [26]). The nested oligonucleotide primer set (1A, 2A, and 3A) used for LMPCR is shown below. Oligonucleotide 1A enabled preferential analysis of the 5′ LTR without interference from signal due to binding sites at the 3′ LTR. The sequence of the linker oligonucleotide used in the LMPCR (1B) is also shown. Oligo, oligonucleotide.