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In vivo DMS footprinting of the M-MuLV 5′ LTR in primary M-MuLV-induced tumor cells. M-MuLV-induced T-lymphoma cells were treated in vivo with DMS and analyzed in the same manner as described for the 43-D and infected Ti-6 cells shown in Fig. 2 and 3. PhosphorImaging was employed to visualize data. In this experiment, the intensity of the entire in vitro lane was uniformly decreased to equalize the amounts of samples loaded and to aid in footprint visualization.
