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. Author manuscript; available in PMC: 2025 Apr 1.
Published in final edited form as: Lab Invest. 2024 Jan 28;104(4):102025. doi: 10.1016/j.labinv.2024.102025

Figure 3: Elevated intraocular pressure in transgenic mice expressing human GDF15.

Figure 3:

To determine the effects of GDF15 on intraocular pressure (IOP), changes in IOP were evaluated in the transgenic mice (in 6 and 10 month-old) expressing human GDF15 under a control of chicken β-actin promoter in comparison of littermate wild type mice. A). Confirming the expressing of human GDF15 in the serum of only the transgenic mice (n=12) as determined by enzyme-linked immunosorbent assay. Human GDF15 levels are robustly (**** P<0.0001; n=12) detected in the serum of only the transgenic mice compared to control mice (n=8). B). Human GDF15 was also detected in the AH of only the transgenic mice based on immunoblot analysis of the pooled sample (n=6 eyes) of AH compared to control mice. C & E). IOP in 6 (n=14 eyes) and 10-month old (n=27 eyes) transgenic mice was significantly elevated (* P<0.011 and P<0.02, respectively) compared to the littermate wild type mice (n=12 & 22 eyes, respectively. D & F). The transgenic (6 and 10 month-old, respectively) and littermate wild type mice exhibit a comparable histological integrity of the AH outflow pathway based on light microscopy imaging. TM; trabecular meshwork, SC; Schlemm’s canal, TG; transgenic, WT; wild type, LC; loading control. Bars indicate image magnification.