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. Author manuscript; available in PMC: 2025 Apr 18.
Published in final edited form as: Cell Chem Biol. 2024 Jan 23;31(4):669–682.e7. doi: 10.1016/j.chembiol.2023.12.020

Figure 2. Genome-scale identification of whiB7-inducing CRISPRi strains in M. smegmatis.

Figure 2.

(A) M. smegmatis whiB7 induction screen workflow.

(B) CFU quantification from the M. smegmatis screen described in panel A.

(C) Volcano plot showing log2 fold-change (L2FC) values and false discovery rates (FDR) for each gene in the M. smegmatis whiB7 induction screen. The expected hit genes ettA and argD are annotated. Hit genes (n=150) were defined as having a L2FC > 2 and FDR < 0.01.

(D) Functional categories of hit genes from the M. smegmatis whiB7 induction screen.