Key resources table: CELL-CHEMICAL-BIOLOGY-D-23-00417R2
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-CD4-APC conjugate | BD Biosciences | Cat# #555349 |
| Mouse monoclonal anti-MHC-I-PE conjugate | Santa Cruz Biotechnology | Cat# sc-32235 PE |
| Mouse monoclonal anti-Actin, clone C4 | Millipore Sigma | Cat# MAB1501R |
| Rabbit polyclonal Anti-HIV-1 Nef antiserum | HIV Reagent Program | Cat# ARP-2949 |
| Bacterial and virus strains | ||
| E. coli DH5α | Thermo Fisher | Cat# 18265017 |
| E. coli Rosetta2(DE3) pLysS | Millipore Sigma | Cat# 71403 |
| Human immunodeficiency virus 1 (HIV-1) | HIV Reagent Program | Cat# ARP-114 |
| Biological samples | ||
| Peripheral blood mononuclear cells | Miltenyi Biotec | Cat# 150-000-450 |
| Chemicals, peptides, and recombinant proteins | ||
| G418 | Thermo Fisher | Cat# 10131027 |
| Puromycin | Millipore Sigma | Cat# P9620 |
| Doxycycline | Takara Bio USA | Cat# 631311 |
| Cell Lysis Buffer | Cell Signaling | Cat# 9803 |
| Protease Inhibitor Cocktail | Millipore Sigma | Cat# P8340 |
| ImmunoCult human CD3/CD28 T cell activator | STEMCELL Technologies | Cat# 10971 |
| Interleukin-2 | BD Biosciences | Cat# 40043B |
| Critical commercial assays | ||
| NanoBRET Ubiquitination Kit | Promega | Cat# ND2690 |
| Lenti-XTM Tet-One System | Takara Bio USA | Cat# 631847 |
| StraightFrom Leukopak PBMC Isolation Kit | Miltenyi Biotec | Cat# 130-123-456 |
| HIV-1 p24 AlphaLISA Assay | Revvity | Cat# AL332C |
| CellTiter-Blue Cell Viability Assay | Promega | Cat# G8080 |
| Experimental models: Cell lines | ||
| CEM-T4 Lymphoblasts | HIV Reagent Program | Cat# ARP-117 |
| 293T Cells | ATCC | Cat# CRL-3216 |
| TZM-bl Reporter Cells | HIV Reagent Program | Cat# ARP-8129 |
| Recombinant DNA | ||
| pET-28a bacterial expression plasmid | Sigma Millipore | Cat# 69864 |
| Software and algorithms | ||
| Prism for statistical analysis, v 10.1 | GraphPad | https://www.graphpad.com/ |
| TraceDrawer for SPR curve fitting | TraceDrawer | https://tracedrawer.com/ |
| Image Studio for immunoblot quantitation | LI-COR Biosciences | https://www.licor.com/bio/image-studio/ |
| BioRender for illustrations | BioRender | https://www.biorender.com/ |
| Other | ||
| HisTrap HP column | Cytiva | Cat# 17524802 |
| HiLoad 16/600 Superdex 75 column | Cytiva | Cat# 28989333 |
| Superdex 75 Increase 10/300 GL | Cytiva | Cat# 29148721 |
| Reichert 4SPR Instrument | Reichert Technologies | https://www.reichertspr.com/en/products/reichert-4spr |
| Carboxymethyl dextran SPR chips | Reichert Technologies | Cat# 13206066 |
| Biotek Cytation 5 Plate Reader | Agilent Technologies | https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202 |
| LI-COR Odyssey CLx Infrared imager for quantitative immunoblot analysis | LI-COR Biosciences | https://www.licor.com/bio/odyssey-dlx/ |