Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ASIC1 | Invitrogen | Cat#PIPA526278 |
| ASIC2a | Invitrogen | Cat#PIPA577729 |
| ASIC3 | ||
| Alexa Fluor 594-conjugated goat anti-rabbit | Invitrogen | A-11076 |
| Alexa Fluor 488-conjygated goat anti-rabbit | Invitrogen | A-11008 |
| Alexa Fluor 594-conjugated goat anti-mouse | Invitrogen | A-11032 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Acetylcholine chloride | Sigma-Aldrich | Cat# A6625 |
| Alexa Fluor 555 | ThermoFisher Scientific | A20501MP |
| Amiloride hydrochloride | Tocris | Cat#0890 |
| Amiloride hydrochloride hydrate | Sigma-Aldrich | Cat#A7410 |
| Ammonium chloride | Sigma-Aldrich | Cat#213330 |
| Antifade Mountant | ThermoFisher Scientific | P36965 |
| APETx2 | Almone labs | Cat#STA-160 |
| Bafilomycin A1 | Tocris | Cat#1334 |
| Cadmium Chloride hydrate | Sigma | Cat#C3141 |
| Caffeine | Sigma-Aldrich | Cat#C0750 |
| Cesium chloride | MilliporeSigma | C3139 |
| Chloroquine | Sigma-Aldrich | Cat#C6628 |
| Citric Acid | Sigma | Cat#C0759 |
| Collagenase | MilliporeSigma | C0130 |
| Diminazene aceturate | Tocris | Cat#6705 |
| Dispase II | Roche | Cat#4942078001 |
| DL-Norepinephrine hydrochloride | Sigma-Aldrich | Cat# A7256 |
| Goat serum | Jackson Immuno Research | 005-000-121 |
| ICI118551 hydrochloride | Sigma-Aldrich | Cat#I127 |
| Isoflurane | Vet One | V1–502017 |
| L-Glutamic acid | Sigma | Cat# G8415 |
| Mefloquine hydrochloride | Sigma-Aldrich | Cat#M2319 |
| Nafamostat mesylate | Sigma-Aldrich | Cat#N0289 |
| Paraformaldehyde (PFA) | Sigma | P6148 |
| PBS | ThermoFisher Scientific | 28374 |
| Psalmotoxin 1 | Tocris | Cat#5042 |
| Quinine | Sigma | Cat#145904 |
| Serotonin hydrochloride | Sigma-Aldrich | Cat#H9523 |
| Serotonin creatinine sulfate monohydrate | Sigma-Aldrich | Cat# H7752 |
| Tetrodotoxin | Tocris | Cat# 1078 |
| VUF10166 | Tocris | Cat#4532 |
| Experimental Models: Animals | ||
| Sprague-Dawley rats | Envigo | Cat#Hsd: |
| C57BL/6J | Jackson Laboratory (USA) | |
| ASIC2+/− mice | Jackson Laboratory (USA) | JAX: # 013126 |
| ASIC3+/− mice | Jackson Laboratory (USA) | JAX: # 013127 |
| ASIC2+/+ mice | In-house breed from ASIC2+/− mice | N/A |
| ASIC2−/− mice | In-house breed from ASIC2+/− mice | N/A |
| ASIC3+/+ mice | In-house breed from ASIC3+/− mice | N/A |
| ASIC3−/− mice | In-house breed from ASIC3+/− mice | N/A |
| Oligonucleotides | ||
| Primers for ASIC2+/+ and ASIC2−/− mice genotyping: | ||
| AGT CCT GCA CGG TGG GAG CTT CTA | Common | |
| GAA GAG GAA GGG AGC CAT GAT GAG | Wild type Forward | |
| TGG ATG TGG AAT GTG TGC GA | Mutant Forward | |
| Primers for ASIC3+/+ and ASIC3−/− mice genotyping: | ||
| GAA CCT GGA AAA CAG AGG CAG GAA GGA T | Wild type Forward | |
| CAG GGA GTA AGA TCT TAT GTA GCC TGG C | Wild type Reverse | |
| CCC TGG GCA CCA GAG TTG AAG GTG TAG C | Mutant Reverse | |
| TGG ATG TGG AAT GTG TGC GA | Mutant Forward | |
| Software and Algorithms | ||
| Clampfit 11 | Molecular Devices | www.moleculardevices.com |
| Deeplabcut | Mathis Laboratory | www.mackenziemathislab.org |
| Fiji | NIH | N/A |
| Matlab | Mathworks | www.mathworks.com |
| Metaflour | Molecular Devices | www.moleculardevices.com |
| Minianalysis | Blue Cell | http://bluecell.co.kr |
| pCLAMP11 | Molecular Devices | www.moleculardevices.com |
| Prism 9 | GraphPad | www.graphpad.com |
| Other | ||
| Cheha supercomputer | UAB | N/A |
| LSM800 | Zeiss | https://www.zeiss.com |
| Nikon A1 Plus | Nikon | https://www.microscope.com |
| Digidata 1440A | Molecular Devices | www.moleculardevices.com |
| Flaming/Brown micropipette puller | Shutter Instruments | Cat# P-97 |
| Glass capillary (thin wall, OD 1.5 mm, filament) | World Precision Instruments | Cat#TW150F-4 |
| High-speed pressure-clamp device | ALA Scientific instruments | https://alascience.com/ |
| IR-2000 | Dagi-MTI | https://dagemti.com |
| Leica CM1860 cryomicrotome | Leica Biosystems | www.leicabiosystems.com |
| Master-8 Stimulator | A.M.P.I | www.ampi.co.il |
| MPC-385 | Sutter instrument | https://www.sutter.com |
| Minipuls 3 Peristaltic Pumps | Gilson | Cat# GFAM00051 |
| Multiclamp 700B | Molecular Devices | www.moleculardevices.com |
| Olympus BX51 | Olympus | www.olympus-lifescience.com |
| Open field box | Maze engineering | https://conductscience.com |
| Recording chamber | Custom made | N/A |
| Syringe filters (0.22 mm) | Research Products International | Cat#256130 |
| Piezoelectric actuator and controller | Physik Instrumente | CAT# E-625.SR, P841.20 |
| Picospritzer II | Parker Hannifin | https://ph.parker.com |
| Tissue anchor | Warner Instruments | Cat#WI 64–1413 |
| Vibration isolation table (63–500 series) | TMC Vibration Control | www.techmfg.com |
| 40x water immersion objective | Olympus | Cat# LUMPLFLN40XW |