Figure 3. Expression patterns of selected proteins.

Panel A shows examples of four rhythmic proteins detected using the 2-harmonic approach, with the rightmost protein showing circadian rhythmicity. Panel B showcases the eight most robust circadian proteins. The individual curves are derived through a series of steps, including log-normalization, mean-filtering, and soft-dtw barycenter computation.³⁴ X-axis is the time relative to Dim Light Melatonin Onset (DLMO). Blue: protein pattern from the 24-hour sleep-wake day in Study 1; orange: protein pattern from the first 24 hours of Constant Routine (CR) in Study 1; purple: protein pattern from the final 15 hours of CR and recovery night in Study 1; green: protein pattern from the first 24 hours of CR in Study 2. Transparent curves represent variations computed using the same process but deviating from the main barycenter by a single subject. Note that not all proteins were assessed in Study 2, which was tested with a different version of the Somalogic panel that did not include them. Additionally, SomaLogic sometimes uses two types of aptamers for the same protein, which is indicated here by a sequence id at the end of the protein’s name for Anterior gradient protein 3 in Panel A.