Skip to main content
. Author manuscript; available in PMC: 2025 Apr 18.
Published in final edited form as: Mol Cell. 2024 Mar 7;84(8):1442–1459.e7. doi: 10.1016/j.molcel.2024.02.013

Figure 4. Constitutively silenced Xp genes are epigenetically distinct.

Figure 4.

(A) Metagene analyses of DNA methylation profiles from single-embryo bisulfite-sequencing across developmental stages. Gene bodies (± 2kb) shown for chrX and representative autosome, chr1. Pat: Paternal chromosome; Mat: maternal chromosome. TSS: Transcription start site; TES: Transcription end site.

(B) Heatmap showing allelic H3K27me3 enrichment on the gene bodies (± 2kb) for 6 gene categories (Cat.) at late 2C and 8C stages60. Comp: composite.

(C) Averaged H3K27me3 enrichment profiles in late 2C embryos from (b). *p=9.31×10−9 in Cat.4, by Wilcoxon Signed-Rank test.

(D) Metagene analysis of chromatin accessibility based on ATAC-seq profiles at promoter regions (TSS ± 2kb) for chr1 and chrX at indicated stages.

(E) Metagene analysis of chromatin accessibility on the gene bodies (± 2kb) for different gene categories at indicated stages based on ATAC-seq profiles.

(F) Representative track views for differential chromatin accessibility and H3K27m3 profiles on Xm versus Xp for Category 4 genes.