Figure 6: Pomiferin delays NBL tumor growth in mouse xenografts.

(A) Stability of pomiferin isopomiferin and ferrostatin-1 (positive control) in mouse plasma across 4 h. Data represent mean ± S.D. of three replicates.
(B) Stability of isopomiferin, pomiferin and 7-ethoxycoumarin (positive control) following 2 h incubation in mouse liver microsomes at 0.5 mg/mL. Data represent mean ± S.D. of three replicates.
(C) Tumor growth across 21 days of treatment with 20 mg/kg pomiferin or vehicle control. (Student’s t-test was performed to assess differences between vehicle and treatment groups; * indicates statistical significance at p<0.05, n=6 animals per group).
(D) Relative volume from mice treated with either isopomiferin (20 mg/kg), pomiferin (20 mg/kg), or vehicle control for 21 days.
(E) Western blot analysis of MYCN in SK-N-Be2 xenografts treated with vehicle, isopomiferin (20 mg/kg) or pomiferin (20 mg/kg) for 14 days.
(F) Mouse body weight across time, following daily treatment with isopomiferin (20 mg/kg), pomiferin (20 mg/kg), or vehicle control for 21 days.